NMD-VCell Neuromuscular Virtual Cell Research Platform Module: Process · Evidence → perturbation → experiment → outcome NMD = neuromuscular disorders

Release 2026.08DMD context observedDMD candidate-conditioned prediction not yet eligible

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Evidence freeze: 3 August 2026 Resource: v1.2.0-measured-dmd-evidence Schema: 1.1 Open release status →

DMD Biological Process Map V1.1

Explore DMD as a biological system—not a list of cell cards.

An evidence-aware skeletal-muscle process graph connects myofibre injury, immune states, myogenic transitions, FAP/ECM remodeling, vascular context and function. Every node and edge declares question identity plus evidence metadata: origin, context match, unit structure, lifecycle and outcome fields.

ScopeDMD skeletal muscle only
EvidenceEvidence-contract metadata; no scalar score
MacrophagesState-based ontology, not M1/M2
LayoutManual hierarchy · seed 42 · not biology

Biological Process Canvas

Damage → inflammation / clearance → regeneration → remodeling → recurrent damage

The feedback loop is a hypothesis-bearing disease system. Arrow existence and evidence metadata matter; node distance does not.

Hand off a missing edge →
Show process nodes and all evidence-labeled biological relationships.
OBSERVED 22COMPUTATIONAL 1MISSING 1
DMD skeletal muscle biological process mapManual hierarchical process map with 24 nodes and 30 evidence-aware edges.DMD genomic lesion → Dystrophin deficiency · OBSERVED · CROSS_SPECIES · AMBIGUOUSDystrophin deficiency → Sarcolemmal / mechanical instability · OBSERVED · CROSS_SPECIES · AMBIGUOUSSarcolemmal / mechanical instability → Ca2+ / cellular stress state · OBSERVED · CROSS_SPECIES · AMBIGUOUSSarcolemmal / mechanical instability → Myofibre injury · OBSERVED · CROSS_SPECIES · AMBIGUOUSMyofibre injury → Degeneration / necrosis · OBSERVED · TARGET_CONTEXT · AMBIGUOUSDegeneration / necrosis → Damage / inflammatory signalling · OBSERVED · TARGET_CONTEXT · AMBIGUOUSDamage / inflammatory signalling → Monocyte / myeloid recruitment · OBSERVED · TARGET_CONTEXT · AMBIGUOUSMonocyte / myeloid recruitment → Phagocytic / clearance macrophage state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSMonocyte / myeloid recruitment → Repair-associated macrophage state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSMonocyte / myeloid recruitment → Fibrosis-associated macrophage state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSFibrosis-associated macrophage state → FAP activation · COMPUTATIONAL · TARGET_CONTEXT · AMBIGUOUSFibrosis-associated macrophage state → Fibrotic stromal / FAP state · COMPUTATIONAL · TARGET_CONTEXT · AMBIGUOUSDegeneration / necrosis → MuSC activation · OBSERVED · TARGET_CONTEXT · AMBIGUOUSMuSC activation → Myogenic proliferation · OBSERVED · SAME_TISSUE · AMBIGUOUSMyogenic proliferation → Myogenic differentiation · OBSERVED · SAME_TISSUE · AMBIGUOUSMyogenic differentiation → Myoblast fusion · OBSERVED · SAME_TISSUE · AMBIGUOUSMyoblast fusion → Regenerated myofibre · MISSING · TARGET_CONTEXT · MISSING_TARGET_CONTEXT_OUTCOMERegenerated myofibre → Myofibre injury · OBSERVED · CROSS_SPECIES · AMBIGUOUSMuSC activation → Regeneration-supportive stromal state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSFAP activation → Regeneration-supportive stromal state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSFAP activation → Fibrotic stromal / FAP state · OBSERVED · TARGET_CONTEXT · AMBIGUOUSFibrotic stromal / FAP state → ECM deposition / remodeling · OBSERVED · TARGET_CONTEXT · AMBIGUOUSECM deposition / remodeling → Fibrosis / adipogenic remodeling · OBSERVED · TARGET_CONTEXT · AMBIGUOUSEndothelial / vascular response → Regenerative niche · OBSERVED · TARGET_CONTEXT · AMBIGUOUSRegeneration-supportive stromal state → Regenerative niche · COMPUTATIONAL · TARGET_CONTEXT · AMBIGUOUSRegenerative niche → Myogenic differentiation · COMPUTATIONAL · TARGET_CONTEXT · AMBIGUOUSMyogenic differentiation → Functional muscle output · MISSING · TARGET_CONTEXT · MISSING_TARGET_CONTEXT_OUTCOMEFibrotic stromal / FAP state → Functional muscle output · MISSING · TARGET_CONTEXT · MISSING_TARGET_CONTEXT_OUTCOMEFunctional muscle output → Myofibre injury · MISSING · TARGET_CONTEXT · MISSING_TARGET_CONTEXT_OUTCOMEMyoblast fusion → Functional muscle output · MISSING · TARGET_CONTEXT · MISSING_TARGET_CONTEXT_OUTCOMEP001DMD genomic lesionOBSERVED/AMBIGUOUSP002Dystrophin deficiencyOBSERVED/AMBIGUOUSP003Sarcolemmal / mechanica…OBSERVED/AMBIGUOUSP004Ca2+ / cellular stress …OBSERVED/AMBIGUOUSP005Myofibre injuryOBSERVED/AMBIGUOUSP006Degeneration / necrosisOBSERVED/AMBIGUOUSP007Damage / inflammatory s…OBSERVED/AMBIGUOUSP008Monocyte / myeloid recr…OBSERVED/AMBIGUOUSP009Phagocytic / clearance …OBSERVED/AMBIGUOUSP010Repair-associated macro…OBSERVED/AMBIGUOUSP011Fibrosis-associated mac…OBSERVED/AMBIGUOUSP012MuSC activationOBSERVED/AMBIGUOUSP013Myogenic proliferationOBSERVED/AMBIGUOUSP014Myogenic differentiationOBSERVED/AMBIGUOUSP015Myoblast fusionOBSERVED/AMBIGUOUSP016Regenerated myofibreOBSERVED/AMBIGUOUSP017FAP activationOBSERVED/AMBIGUOUSP018Regeneration-supportive…OBSERVED/AMBIGUOUSP019Fibrotic stromal / FAP …OBSERVED/AMBIGUOUSP020ECM deposition / remode…OBSERVED/AMBIGUOUSP021Fibrosis / adipogenic r…OBSERVED/AMBIGUOUSP022Endothelial / vascular …OBSERVED/AMBIGUOUSP023Regenerative nicheCOMPUTATIONAL/AMBIGUOUSP024Functional muscle outputMISSING/MISSING_TARGET_CONTEXT_OUTCOME

Reading rule: Node coordinates encode a reproducible reading order only; position and distance are not biological evidence.

Evidence Coverage

Every source has a mapping state and a next gate.

Coverage counts map source objects to process nodes and edges; they do not measure effect size, causality or confidence.

9source objects
9mapped to the process
3sources needing a next gate
Traceablemapping policy
SourceMapping stateMap linksReadinessNext gateRoute
GSE272233DMD-locus correction transcript referenceRELEASED_REFERENCE3 nodes · 0 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object
PRJNA772047Human DMD patient skeletal muscle referenceRELEASED_REFERENCE7 nodes · 2 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object
GSE288958Human DMD/BMD/control muscle snRNA-seq and spatial contextgse288958_sample_aware_module_context_v01CONTEXT_READY9 nodes · 4 edgescontext readyUse the read-only sample-aware reference with Normal_2, Scrublet and annotation-disagreement sensitivities. Unfiltered droplets or author contamination estimates are needed only to close ambient RNA; exact author artifacts are needed only for an author-exact reprocessing claim.Source object
Sample-aware context object · Formal QC gate · Reference audit · Reference audit JSON · Context brief · Stable module table · Candidate context triage
GSE277637DMD iPSC-derived skeletal-muscle organoid scRNA-seqgse277637_external_dmd_organoid_validation_v01CONTEXT_READY4 nodes · 0 edgescontext readyFreeze line-aware sample metadata, preserve one-line-one-unit aggregation, publish source checksum and license record before public matrix release.Source object
Cross-dataset metrics · Line-aware context object
GSE293514Human myoblast fusion CRISPR screen and CROP-seq contextIDENTITY_BLOCKED2 nodes · 2 edgesintake gateRecover author-ordered feature table, guide reference, exact cell metadata and processing contract; checksum every returned file before any expression transfer.Source object
GSE52529Human myogenic differentiation time courseRELEASED_REFERENCE3 nodes · 5 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object
DMD-MUSCLE-REVIEWDMD skeletal-muscle disease mechanism anchorCURATED_CONTEXT5 nodes · 5 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object
DMD-SPATIAL-CONTEXTDMD spatial degeneration, regeneration and fibrosis contextCURATED_CONTEXT15 nodes · 17 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object
STUDY-CARD-MANIFEST21 governed DMD Study Card draftsREGISTRY_HANDOFF1 nodes · 5 edgescurated / releasedNo additional intake gate; retain the declared contextual or curated claim ceiling.Source object

Current actionable gap: GSE277637 maps to four target-context process nodes. GSE288958 is now CONTEXT_READY with a sample-aware context object, read-only QC, donor-wise Scrublet and independent annotation-disagreement audits; its 1,967-cell raw-to-object gap, ambient-RNA boundary and author-exact reprocessing gap remain visible. Neither source establishes a cell-state transition, macrophage/FAP communication, candidate perturbation response or function.

Evidence Inspector

Click a node or edge to inspect its claim ceiling.

Spatial co-localization, ligand–receptor inference and trajectory references retain explicit evidence metadata. They never silently become causal edges.

State transition contract

The first computable transition layer is myogenic; DMD-conditioned transitions remain NOT ESTIMABLE.

Open measured trajectory →
Observed referenceMuSC → proliferation → differentiation → fusion

GSE52529 and GSE293514 provide unperturbed time and healthy-myoblast context.

Missing DMD outcomeDMD myogenic perturbation → regenerated fibre → function

Candidate-level DMD outcomes have not yet been measured; missing is not negative.

Future outputP(state A → B) + uncertainty + abstention

Only after donor, time, perturbation and functional outcomes are frozen and measured.

Claim boundary

Mechanism → experiment → outcome → claim update

DMD Biological Process Map V1.0 is an evidence-aware mechanistic map. It is not a causal network, perturbation-conditioned virtual-cell prediction, patient simulation, treatment ranking or clinical decision system. Missing is not negative, and layout is not biology.