What it proves
A physical-time human myogenic differentiation reference exists across 0, 24, 48 and 72 hours.
single cell · Single-cell expression, state and pseudotime
Dataset Card · nmd-vcell-dataset-registry-record/1.0
Human myogenic differentiation time course
A versioned, citable account of what was measured, which task this object may support and which prediction claims remain blocked.
01SourceGSE52529
→02Typed contextprimary skeletal myoblast differentiation
→03Qualified roleBuild an unperturbed human-myogenic state reference
→04DMD prediction outcomeNot qualified
What it cannot prove
It cannot simulate disease progression, aging, treatment response or perturbation-conditioned fate.
Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility.
Best model use
Use as an unperturbed trajectory/state reference and timing scaffold.
Build an unperturbed human-myogenic state reference
Next experiment handoff
Add candidate perturbation at defined lineage times with matched DMD/control cells and frozen fate endpoints.
No perturbation-conditioned trajectory and no longitudinal DMD state
Identity & access
Source receipt
- Accession
- GSE52529
- Licence
- Source repository and package terms apply; verify at accession
- Source verification
- required
- Stable page
/resource/dataset/gse52529/
Biological context
What it represents
- Disease / context
- Non-disease trajectory reference
- Species
- Homo sapiens
- Tissue / model
- primary skeletal myoblast differentiation
- Cell type
- Primary human skeletal myoblast
- Donor metadata
- not suitable for unseen donor perturbation
Measurement design
What was measured
- Modality
- single-cell expression
- Observation unit
- single cell
- Size
- 271 cells
- Count state
- 271 cells
- Endpoint
- Single-cell expression, state and pseudotime
Perturbation design
Intervention contract
- Perturbation
- None
- Dose
- unperturbed
- Timepoints
- 0, 24, 48 and 72 hours
- Direct DMD candidate outcome
- No
Replication & provenance
Receipt completeness
- Biological replicates
- source package required
- Technical replicates
- source package required
- Batch / platform
- source package required
- Raw hashes
- bioconductor source package checksummed
- Processed hashes
- derived trajectory objects available
- Analysis hashes
- derived tables in resource manifest
Benchmark partition
Permitted computational use
Unperturbed trajectory reference only
G4_unperturbed_state_reference_only
- Leakage grouping
- trajectory reference no perturbation holdout
- Donor holdout
- No
- Disease-state holdout
- No
- Prospective holdout
- No
Readiness decision
Build an unperturbed human-myogenic state reference
No perturbation-conditioned trajectory and no longitudinal DMD state
Associated resources