{
  "registry_object_schema": "nmd-vcell-dataset-registry-record/1.0",
  "dataset_id": "GSE52529",
  "accession": "GSE52529",
  "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52529",
  "stable_url": "/resource/dataset/gse52529/",
  "json_url": "/resource/api/v1.1/datasets/gse52529.json",
  "title": "Human myogenic differentiation time course",
  "source_identity": {
    "accession": "GSE52529",
    "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE52529",
    "license_status": "Source repository and package terms apply; verify at accession",
    "source_verification_required": true
  },
  "biological_context": {
    "disease_or_context": "Non-disease trajectory reference",
    "species": "Homo sapiens",
    "tissue_or_model": "primary skeletal myoblast differentiation",
    "cell_type": "Primary human skeletal myoblast",
    "donor_age_sex_stage_state": "not_suitable_for_unseen_donor_perturbation"
  },
  "measurement_design": {
    "modality": "single-cell expression",
    "observation_unit": "single cell",
    "cell_count_or_sample_size": "271 cells",
    "cell_count_state": "271_cells",
    "endpoint": "Single-cell expression, state and pseudotime"
  },
  "perturbation_design": {
    "perturbation": "None",
    "dose": "unperturbed",
    "timepoints": "0, 24, 48 and 72 hours",
    "direct_dmd_candidate_perturbation_outcome": false,
    "direct_dmd_candidate_perturbation_truth": false
  },
  "replication_and_batch": {
    "biological_replicate_state": "source_package_required",
    "technical_replicate_state": "source_package_required",
    "batch_platform_state": "source_package_required"
  },
  "file_hash_chain": {
    "raw": "bioconductor_source_package_checksummed",
    "processed": "derived_trajectory_objects_available",
    "analysis": "derived_tables_in_resource_manifest"
  },
  "benchmark_partitioning": {
    "leakage_grouping": "trajectory_reference_no_perturbation_holdout",
    "permitted_benchmark_use": [
      "G4_unperturbed_state_reference_only"
    ],
    "donor_holdout_qualified": false,
    "disease_state_holdout_qualified": false,
    "prospective_holdout_qualified": false
  },
  "ai_readiness_state": "TRAJECTORY_REFERENCE_ONLY",
  "current_ai_role": "Build an unperturbed human-myogenic state reference",
  "decision_blocker": "No perturbation-conditioned trajectory and no longitudinal DMD state",
  "research_value": {
    "value_schema": "nmd-vcell-dataset-research-value/1.0",
    "what_it_proves": "A physical-time human myogenic differentiation reference exists across 0, 24, 48 and 72 hours.",
    "what_it_cannot_prove": "It cannot simulate disease progression, aging, treatment response or perturbation-conditioned fate.",
    "best_model_use": "Use as an unperturbed trajectory/state reference and timing scaffold.",
    "next_experiment_handoff": "Add candidate perturbation at defined lineage times with matched DMD/control cells and frozen fate endpoints.",
    "claim_boundary": "Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility."
  },
  "boundary": "Dataset registration does not convert context evidence into DMD perturbation-response outcome."
}