Human myoblast fusion CRISPR screen

What it proves

Healthy-human-myoblast knockout perturbation effects can be read against a fusion endpoint and named validation context.

screen-level and selected validation objects · Fusion screen, individual validation and grouped CROP-seq myogenesis state

What it cannot prove

It cannot establish that the same candidate rescues or worsens a DMD muscle phenotype.

Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility.

Best model use

Use for muscle-context coverage, endpoint compatibility and external-context stress tests.

Qualify muscle-context coverage and endpoint compatibility

Next experiment handoff

Convert candidate coverage into a DMD/control myogenic Study Card with fusion and molecular endpoints.

Not an independent candidate perturbation in a DMD model

Identity & access

Source receipt

Accession

GSE293514

Licence

Source repository and publication terms apply; verify at accession

Source verification

required

Stable page

/resource/dataset/gse293514/

Biological context

What it represents

Disease / context

Muscle context; not DMD rescue

Species

Homo sapiens

Tissue / model

human myoblast fusion screen

Cell type

Human myoblast

Donor metadata

source publication required

Measurement design

What was measured

Modality

screen endpoint and validation workbooks

Observation unit

screen-level and selected validation objects

Size

7,197 screen genes; 250 hits; 125 individually validated genes

Count state

not released as cell population truth

Endpoint

Fusion screen, individual validation and grouped CROP-seq myogenesis state

Perturbation design

Intervention contract

Perturbation

Knockout screen

Dose

knockout screen; dose not harmonized in current object

Timepoints

Publication endpoint

Direct DMD candidate outcome

No

Replication & provenance

Receipt completeness

Biological replicates

source publication required

Technical replicates

source publication required

Batch / platform

source publication required

Raw hashes

source workbooks checksummed when in release

Processed hashes

derived context screen objects available

Analysis hashes

derived tables in resource manifest

Benchmark partition

Permitted computational use

Observed healthy-myoblast functional perturbation reference (Stage B0), not DMD replication

• L3a_context_screen_interpretation

Leakage grouping

context screen only not training truth

Donor holdout

No

Disease-state holdout

No

Prospective holdout

No