{
  "registry_object_schema": "nmd-vcell-dataset-registry-record/1.0",
  "dataset_id": "GSE293514",
  "accession": "GSE293514",
  "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293514",
  "stable_url": "/resource/dataset/gse293514/",
  "json_url": "/resource/api/v1.1/datasets/gse293514.json",
  "title": "Human myoblast fusion CRISPR screen",
  "source_identity": {
    "accession": "GSE293514",
    "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293514",
    "license_status": "Source repository and publication terms apply; verify at accession",
    "source_verification_required": true
  },
  "biological_context": {
    "disease_or_context": "Muscle context; not DMD rescue",
    "species": "Homo sapiens",
    "tissue_or_model": "human myoblast fusion screen",
    "cell_type": "Human myoblast",
    "donor_age_sex_stage_state": "source_publication_required"
  },
  "measurement_design": {
    "modality": "screen endpoint and validation workbooks",
    "observation_unit": "screen-level and selected validation objects",
    "cell_count_or_sample_size": "7,197 screen genes; 250 hits; 125 individually validated genes",
    "cell_count_state": "not_released_as_cell_population_truth",
    "endpoint": "Fusion screen, individual validation and grouped CROP-seq myogenesis state"
  },
  "perturbation_design": {
    "perturbation": "Knockout screen",
    "dose": "knockout screen; dose not harmonized in current object",
    "timepoints": "Publication endpoint",
    "direct_dmd_candidate_perturbation_outcome": false,
    "direct_dmd_candidate_perturbation_truth": false
  },
  "replication_and_batch": {
    "biological_replicate_state": "source_publication_required",
    "technical_replicate_state": "source_publication_required",
    "batch_platform_state": "source_publication_required"
  },
  "file_hash_chain": {
    "raw": "source_workbooks_checksummed_when_in_release",
    "processed": "derived_context_screen_objects_available",
    "analysis": "derived_tables_in_resource_manifest"
  },
  "benchmark_partitioning": {
    "leakage_grouping": "context_screen_only_not_training_truth",
    "permitted_benchmark_use": [
      "L3a_context_screen_interpretation"
    ],
    "donor_holdout_qualified": false,
    "disease_state_holdout_qualified": false,
    "prospective_holdout_qualified": false
  },
  "ai_readiness_state": "CONTEXT_SCREEN_ONLY",
  "current_ai_role": "Qualify muscle-context coverage and endpoint compatibility",
  "decision_blocker": "Not an independent candidate perturbation in a DMD model",
  "research_value": {
    "value_schema": "nmd-vcell-dataset-research-value/1.0",
    "what_it_proves": "Healthy-human-myoblast knockout perturbation effects can be read against a fusion endpoint and named validation context.",
    "what_it_cannot_prove": "It cannot establish that the same candidate rescues or worsens a DMD muscle phenotype.",
    "best_model_use": "Use for muscle-context coverage, endpoint compatibility and external-context stress tests.",
    "next_experiment_handoff": "Convert candidate coverage into a DMD/control myogenic Study Card with fusion and molecular endpoints.",
    "claim_boundary": "Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility."
  },
  "boundary": "Dataset registration does not convert context evidence into DMD perturbation-response outcome."
}