What it proves
CRISPRa single and pair perturbation design can be calibrated in a public single-cell method reference.
single cell · Single-cell gene expression
Dataset Card · nmd-vcell-dataset-registry-record/1.0
CRISPRa single and combinatorial calibration reference
A versioned, citable account of what was measured, which task this object may support and which prediction claims remain blocked.
01SourceGSE133344
→02Typed contextK562 method-calibration reference
→03Qualified roleCalibrate activation and combination task design
→04DMD prediction outcomeNot qualified
What it cannot prove
It cannot support muscle/DMD overexpression claims or infer combination benefit for current candidates.
Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility.
Best model use
Use for activation-design and pair-task calibration outside the DMD biological claim space.
Calibrate activation and combination task design
Next experiment handoff
Move activation or pair hypotheses into a muscle/DMD-specific assay before interpreting biological effect.
No matched muscle or DMD activation response for current candidates
Identity & access
Source receipt
- Accession
- GSE133344
- Licence
- Source repository and publication terms apply; verify at accession
- Source verification
- required
- Stable page
/resource/dataset/gse133344/
Biological context
What it represents
- Disease / context
- Non-muscle method calibration
- Species
- Homo sapiens
- Tissue / model
- K562 method-calibration reference
- Cell type
- K562
- Donor metadata
- not a donor generalization set
Measurement design
What was measured
- Modality
- single-cell perturbation expression
- Observation unit
- single cell
- Size
- 287 perturbations; 112 singles; 132 pairs
- Count state
- public method reference
- Endpoint
- Single-cell gene expression
Perturbation design
Intervention contract
- Perturbation
- CRISPRa singles and pairs
- Dose
- CRISPRa singles and pairs; dose not harmonized in current object
- Timepoints
- Study endpoint
- Direct DMD candidate outcome
- No
Replication & provenance
Receipt completeness
- Biological replicates
- source publication required
- Technical replicates
- source publication required
- Batch / platform
- source publication required
- Raw hashes
- upstream accession required
- Processed hashes
- processed feature table checksummed
- Analysis hashes
- derived calibration objects available
Benchmark partition
Permitted computational use
Method calibration only; not muscle/DMD overexpression evidence
G7_method_calibration_reference_only
- Leakage grouping
- method calibration only not dmd training
- Donor holdout
- No
- Disease-state holdout
- No
- Prospective holdout
- No
Readiness decision
Calibrate activation and combination task design
No matched muscle or DMD activation response for current candidates
Associated resources