{
  "registry_object_schema": "nmd-vcell-dataset-registry-record/1.0",
  "dataset_id": "GSE133344",
  "accession": "GSE133344",
  "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133344",
  "stable_url": "/resource/dataset/gse133344/",
  "json_url": "/resource/api/v1.1/datasets/gse133344.json",
  "title": "CRISPRa single and combinatorial calibration reference",
  "source_identity": {
    "accession": "GSE133344",
    "permanent_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE133344",
    "license_status": "Source repository and publication terms apply; verify at accession",
    "source_verification_required": true
  },
  "biological_context": {
    "disease_or_context": "Non-muscle method calibration",
    "species": "Homo sapiens",
    "tissue_or_model": "K562 method-calibration reference",
    "cell_type": "K562",
    "donor_age_sex_stage_state": "not_a_donor_generalization_set"
  },
  "measurement_design": {
    "modality": "single-cell perturbation expression",
    "observation_unit": "single cell",
    "cell_count_or_sample_size": "287 perturbations; 112 singles; 132 pairs",
    "cell_count_state": "public_method_reference",
    "endpoint": "Single-cell gene expression"
  },
  "perturbation_design": {
    "perturbation": "CRISPRa singles and pairs",
    "dose": "CRISPRa singles and pairs; dose not harmonized in current object",
    "timepoints": "Study endpoint",
    "direct_dmd_candidate_perturbation_outcome": false,
    "direct_dmd_candidate_perturbation_truth": false
  },
  "replication_and_batch": {
    "biological_replicate_state": "source_publication_required",
    "technical_replicate_state": "source_publication_required",
    "batch_platform_state": "source_publication_required"
  },
  "file_hash_chain": {
    "raw": "upstream_accession_required",
    "processed": "processed_feature_table_checksummed",
    "analysis": "derived_calibration_objects_available"
  },
  "benchmark_partitioning": {
    "leakage_grouping": "method_calibration_only_not_dmd_training",
    "permitted_benchmark_use": [
      "G7_method_calibration_reference_only"
    ],
    "donor_holdout_qualified": false,
    "disease_state_holdout_qualified": false,
    "prospective_holdout_qualified": false
  },
  "ai_readiness_state": "METHOD_CALIBRATION_ONLY",
  "current_ai_role": "Calibrate activation and combination task design",
  "decision_blocker": "No matched muscle or DMD activation response for current candidates",
  "research_value": {
    "value_schema": "nmd-vcell-dataset-research-value/1.0",
    "what_it_proves": "CRISPRa single and pair perturbation design can be calibrated in a public single-cell method reference.",
    "what_it_cannot_prove": "It cannot support muscle/DMD overexpression claims or infer combination benefit for current candidates.",
    "best_model_use": "Use for activation-design and pair-task calibration outside the DMD biological claim space.",
    "next_experiment_handoff": "Move activation or pair hypotheses into a muscle/DMD-specific assay before interpreting biological effect.",
    "claim_boundary": "Dataset value is task-specific; no Dataset Card alone is evidence of candidate efficacy, patient response or clinical utility."
  },
  "boundary": "Dataset registration does not convert context evidence into DMD perturbation-response outcome."
}