NMD-VCell Virtual Cell Evidence Engine See what is known, what is uncertain, and what to test next Module: Study design · evidence-to-experiment workflow NMD = neuromuscular disorders

Current limit: the site can compare existing evidence, but no candidate has yet been independently repeated in a DMD muscle model.

Research evidence only 21 observed HepG2 perturbations 0 independent DMD replications Boundary & release
Observed HepG2 perturbations with limited external myogenic context No validated DMD perturbation prediction v1.0.0-database-resource Frozen 25 Jul 2026 Schema 1.1 Model ridge-safe-v2.3 Benchmark repeated-fold-v2.2 Build EA-20260730-33 DOI pending Open evidence boundary →

Plan an experiment

Study design

Design the next experiment, then review the available Study Cards.

Search a gene, generate a structured Study Card draft, or open one of three recommended examples. The planner does not rank candidates or upgrade evidence levels.

Step 1 of 5

Choose a gene; shared links restore the selection automatically.

Recommended examples: ZNF133 · CPEB1 · GFOD2.

Five-step experiment planner

Answer four more questions to create a Study Card.

What this means: the card records the question, cell model, main outcome and success rule before results are known.

Planner visual builder

Turn one gene into a bounded experiment route.

The planner is a preregistration-style experimental workflow: it resolves a gene, shows the frozen evidence state, exposes the blocking gap, selects the current assay route and lists the registration fields still open.

BoundaryThis builder does not rank candidates, change evidence levels, compute target scores, infer therapeutic direction or make disease-prediction claims.
Draft Study Cards21versioned Study Cards
Action classes5current next-step categories
L3b replications0same-perturbation evidence absent
Prediction statusP0P1/P2 remain locked
1Resolve geneHGNC identifier and typed record
2Evidence stateL1/L2/L3a fields only
3Blocking gapgap code stays explicit
4Assay routeaction-specific scaffold
5Register draftrequires frozen fields

Current Study Card action map

Test in human myogenic cells2 Study Cards
Run an independent human myogenic perturbation experiment.
Confirm the gene is detectable4 Study Cards
Confirm target detectability before perturbation escalation.
Review safety and biological context7 Study Cards
Resolve dependency, toxicity or context risks before escalation.
Reproduce the result independently7 Study Cards
Repeat the source-state analysis in an independent frozen workflow.
Pause until external evidence is audited1 Study Card
Import and audit the reported external evidence before changing state.

Stable governed records

Versioned Study Cards grouped by current action

All 21 routes are static, shareable and tied to v1.0.0-database-resource, schema 1.1 and build EA-20260730-33. JSON, YAML and TSV exports are available on every card.

Test in human myogenic cells

2 Study Cards

Run an independent human myogenic perturbation experiment.

2

Confirm the gene is detectable

4 Study Cards

Confirm target detectability before perturbation escalation.

4

Review safety and biological context

7 Study Cards

Resolve dependency, toxicity or context risks before escalation.

7

Reproduce the result independently

7 Study Cards

Repeat the source-state analysis in an independent frozen workflow.

7

Pause until external evidence is audited

1 Study Card

Import and audit the reported external evidence before changing state.

1