object_id	card_schema	card_version	resource_release	evidence_freeze	interface_build	gene	card_type	current_action_code	current_action	lifecycle	decision_blocking_gap	highest_missing_evidence_layer	secondary_gaps	frozen_evidence_snapshot	unresolved_question	biological_context	perturbation_modality	comparator_and_controls	primary_endpoint	secondary_endpoints	primary_estimand	state_transition_design	perturbation_direction_rationale	minimally_important_effect	negligible_effect_margin	suggested_sample_size_range	randomisation_unit	blocking_factors	statistical_model	multiple_testing_family	missing_data_rule	assay_qc_thresholds	guide_concordance_rule	donor_replication_rule	estimated_time_band	estimated_cost_band	preregistration_status	inferential_unit	evidence_import_requirements	planning_fields_not_applicable	stop_rules	escalation_rule	evidence_transition	data_release_plan	boundary	stable_url	exports
SC:v1.0.0-database-resource:ADAM10:1.5-DRAFT	nmd-vcell-study-card/1.5	1.5	v1.0.0-database-resource	2026-07-25	EA-20260729-15	ADAM10	computational_replication	computational_replication	Computational replication	{"state":"DRAFT","revision":1,"immutable":false,"registered_at":null,"supersedes":null,"immutable_after_registration":true}	{"code":"GAP-07","label":"Independent computational replication is absent"}	L4_dmd_functional_validation	GAP-02 independent muscle-context perturbation | GAP-03 DMD-relevant functional validation	{"snapshot_schema":"nmd-vcell-study-card-frozen-evidence-snapshot/1.0","source_record":"gene/ADAM10","highest_supported_level":"L2_observed_same_context_perturbation","highest_assessed_level":"L3a_external_context_screen","observed_hepg2_perturbation":{"assessment_status":"assessed","support_status":"supported","observed_cells":161},"external_context_screen":{"assessment_status":"assessed","support_status":"context_evidence_present","hit":false,"effect_size":-0.075195,"fdr":0.999999,"interpretation":"assessed_no_current_hit"},"dmd_prior":{"integrated_prior_direction":"up","source_direction_state":"conflicted","source_agreement_proportion":0.5,"agreeing_sources":2,"assessed_sources":4,"coverage_state":"4_of_4_sources","uncertainty_state":"direction_conflict_retained"},"context_metrics":{"skeletal_muscle_median_tpm":3.1162,"depmap_median_gene_effect":0.0120927686971809,"moderate_dependency_flag":false},"visual_boundary":"Descriptive frozen evidence snapshot only; no score, rank, cluster, prediction claim or intervention recommendation is generated."}	Does the ADAM10 evidence pattern replicate in an independent dataset under a frozen analysis plan?	At least one independent dataset with declared tissue/cell state, disease status and gene-mapping coverage.	No new wet-lab perturbation is nominated; rerun the frozen direction, pathway and robustness analyses.	Frozen null and simple baselines. | Leave-one-source-out analysis. | Negative-control genes or permuted labels. | Complete unmatched-identifier report.	Predeclared gene-level direction or effect estimate in the independent dataset.	Pathway concordance. | Leave-one-source-out robustness. | Coverage and null-result diagnostics.	Independent-dataset gene effect and uncertainty under the frozen contrast.	{"scientific_object":"perturbation × cell state × disease context × time × phenotype","mechanism_hypothesis":"Required before registration: state the proposed early molecular mediator, the expected cell-state transition and the downstream functional consequence.","cell_context_fields":{"disease_background":"Required before registration: healthy, DMD or isogenic corrected.","myogenic_state":"Required before registration: proliferating myoblast, early differentiation, fusion or maturing myotube.","donor_or_isogenic_pair":"Required before registration; preserve donor-specific estimates."},"timepoint_plan":[{"window":"6–12 h","role":"early molecular or signalling response","status":"planning_default_requires_assay_calibration"},{"window":"24–48 h","role":"regulatory program and cell-state transition","status":"planning_default_requires_assay_calibration"},{"window":"4–7 d","role":"differentiation and functional phenotype","status":"planning_default_requires_assay_calibration"}],"endpoint_domains":{"target_engagement":["mRNA","protein where validated","perturbation efficiency"],"functional":["fusion","morphology","membrane integrity","calcium","contraction"],"safety":["viability","proliferation","differentiation blockade","global stress"],"replication":["reagent","donor or isogenic pair","future batch"]},"cell_cell_consequence":{"current_status":"not_assessed","future_levels":["conditioned medium","two-cell co-culture","three-dimensional muscle model","spatial perturbation model"]},"response_archetype":{"current_status":"not_assessed","allowed_values":["robust_responder","dmd_specific_responder","donor_variable","state_specific","toxic_responder","null_with_equivalence_margin","discordant","qc_failure","inconclusive"]}}	{"selected_hypothesis":"unresolved_requires_registration","allowed_hypotheses":["activation","inhibition","bidirectional_exploration","direction_not_identifiable"],"required_justification":"State whether the disease-associated direction is hypothesized as causal, compensatory or accompanying, and preserve the opposite-direction alternative. DMD direction and counteralignment never choose an intervention automatically."}	Required before registration; derive from assay biology or a justified pilot and store the numeric value with units.	Required before interpreting a null result; store a symmetric or asymmetric numeric margin with units.	One or more independent datasets; donor/sample adequacy must be justified from the source design rather than a generic target count.	Independent donor/sample/study unit defined by the source dataset.	Study/donor. | Batch. | Cell state. | Dataset-specific covariates.	Dataset-appropriate pseudobulk or aggregate model with donor/sample as the inferential unit.	Frozen gene and pathway families with declared adjustment method.	Define exclusions before unblinding; report all missing units and reasons; do not single-impute primary outcomes without a prespecified sensitivity analysis.	Minimum donor/sample coverage. | Gene detectability. | Frozen mapping and exclusion thresholds.	Not applicable unless the independent dataset contains multiple perturbation reagents.	A result must persist beyond a single donor/sample and disclose leave-one-unit-out sensitivity.	1–4 weeks after data access; planning estimate only.	Institution- and assay-dependent; obtain a local itemised quote before registration.	draft_requires_direction_rationale_numeric_effect_margin_sample_size_and_qc_thresholds	Independent biological replicate or independently generated perturbation unit; cells within one aggregate are not inferential replicates.			Stop or classify as infeasible if a required numeric QC threshold fails. | Do not interpret a nonsignificant result as no material effect without a negligible-effect interval. | Do not change canonical candidate status automatically; require governed review.	Escalate from L2 to L3b only after independent context-matched perturbation replication; L4 requires replicated DMD-relevant muscle evidence.	Current evidence state → predeclared independent test → governed evidence-level review.	Release the frozen card, protocol identifiers, analysis code, complete denominators and results irrespective of direction; never overwrite the registered card.	This Study Card is an evidence-gated design scaffold, not a protocol, power calculation, safety claim, prediction or therapeutic recommendation.	study-card/ADAM10	{"json":"api/v1.1/study-cards/ADAM10.json","yaml":"downloads/study-cards/ADAM10.yaml","tsv":"downloads/study-cards/ADAM10.tsv"}