{
  "schema": "nmd-vcell-registered-singlecell-summary/2.0",
  "release": "multidisease_release_v02",
  "generated_at": "2026-08-05T16:28:48+0800",
  "status": "ANALYZED_WITH_DIRECTIONAL_SMALL_N_BOUNDARY",
  "datasets": {
    "GSE303359": {
      "status": "ANALYZED",
      "libraries": 8,
      "biological_lines": 4,
      "lines_per_genotype": 2,
      "raw_cells": 41486,
      "retained_cells": 40289,
      "statistical_strategy": "line-blocked voom-limma pseudobulk with repeated baseline/injury measures",
      "visualization_correction": "limma_removeBatchEffect_on_PCs_preserving_genotype_by_treatment",
      "leading_module_interaction": {
        "module": "DUX4_target",
        "estimate": 0.3923,
        "ci_low": -0.8337,
        "ci_high": 1.6183,
        "p_value": 0.44,
        "fdr": 0.8834
      }
    },
    "GSE290980": {
      "status": "ANALYZED",
      "libraries": 8,
      "biological_lines": 4,
      "lines_per_genotype": 2,
      "raw_cells": 39179,
      "retained_cells": 37157,
      "statistical_strategy": "technical-library aggregation followed by line-level directional effects without calibrated p-values",
      "visualization_correction": "within_line_technical_library_PC_centering_preserving_line_centroids",
      "largest_composition_shift": {
        "marker_state": "Motor_neuron_like",
        "proportion_difference": -0.3387,
        "directional_consistency": "MIXED"
      },
      "kif5a": "KIF5A line-level log2 effect -0.294 (DOWN_ALL_PAIRWISE)"
    }
  },
  "candidate_update": {
    "promoted_to_validated_target": 0,
    "interpretation": "The single-cell extensions localize states and refine experiment design; they do not promote any therapeutic target or create a cross-disease score."
  },
  "claim_boundary": "n=2 biological lines per genotype; directional evidence only; no efficacy, clinical or patient-calibrated claims"
}