# GSE277637 line-aware context object

- Object ID: `CONTEXT:GSE277637:LINE_AWARE_DMD_ORGANOID:1.0`
- Status: **CITABLE_LOCAL_CONTEXT_OBJECT_NOT_PUBLIC_MATRIX_RELEASE**
- Official source: [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277637](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE277637)
- Checked: 2026-08-09

## Scope

DMD organoid disease-axis heterogeneity stress test. The inferential unit is **iPSC-derived line/sample; cells are nested observations, not independent biological replicates**.

## Sample units

| Sample | Condition | Cells | Detected genes | Median UMIs/cell |
|---|---|---:|---:|---:|
| DMD1 | DMD | 2017 | 21759 | 1329 |
| DMD2 | DMD | 2771 | 22862 | 1065 |
| DMD3 | DMD | 3206 | 23028 | 994 |
| WT | HEALTHY | 2486 | 23034 | 1492.5 |

All three DMD lines share one WT control. Cells are not independent biological replicates, and no cell-level P-value is published by this object.

## Claim ceiling

Supports donor/line-aware DMD disease context and organoid heterogeneity stress testing only. It does not support candidate perturbation response, therapeutic efficacy, clinical prediction or a calibrated DMD predictor.

Direct DMD candidate perturbation truth remains **0/21**.