{
  "schema": "nmd-vcell-gse272233-replicate-aware-reactome/1.0",
  "generated": "2026-08-03T21:05:33+0800",
  "source": {
    "accession": "GSE272233",
    "accession_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE272233",
    "matrix_sha256": "072e1398918140af4898cc895dcbd6c72c28beb39353834063e457f9d0161eee",
    "reactome_sha256": "89983d5c1f0af11c52edfeee7323eb425580ac6281d387a528562ab1787ce56b",
    "local_paths_exposed": false
  },
  "design": {
    "samples": 21,
    "groups": {
      "WT": 3,
      "Dup2": 3,
      "Dup2_corrected": 3,
      "Dup2_9": 3,
      "Dup2_9_corrected": 3,
      "Dup8_9": 3,
      "Dup8_9_corrected": 3
    },
    "biological_repeats_per_group": 3,
    "retained_genes": 22230,
    "tested_reactome_pathways": 1559,
    "camera_inter_gene_correlation": 0.01
  },
  "results": {
    "camera_rows": 9354,
    "background_reversal_rows": 4677,
    "consensus_dual_reversal_pathways_all_3_backgrounds": 0,
    "replicated_dual_reversal_pathways_2plus_backgrounds": 0,
    "background_specific_dual_reversal_pathways": 24,
    "background_specific_dual_reversal_counts": {
      "dup2": 0,
      "dup2_9": 0,
      "dup8_9": 24
    },
    "meta_reversal_fdr_lt_0_05": 492,
    "heterogeneous_pathways_q_p_lt_0_10_i2_ge_50": 788
  },
  "claim_boundary": {
    "allowed": "Replicate-aware pathway effects after DMD-locus correction and exploratory disease-to-correction reversal within each mutation background.",
    "blocked": "Candidate-gene perturbation effects in DMD cells, therapeutic efficacy, and candidate-level DMD virtual-cell prediction.",
    "caveats": [
      "WT is not isogenic to each disease background; disease-vs-WT reversal is exploratory.",
      "Corrected clones may retain clone-specific effects.",
      "Fixed-effect reversal meta-analysis uses moderated standard errors and disease point-estimate orientation; it is exploratory.",
      "Reactome pathways overlap and are not independent hypotheses."
    ]
  },
  "resource_release": "v1.2.0-measured-dmd-evidence",
  "interface_build": "EA-20260804-49"
}