{
  "schema": "nmd-vcell-external-dmd-pathway-reversal/1.0",
  "generated": "2026-08-03T21:18:39+0800",
  "datasets": {
    "PRJNA772047": {
      "samples": 5,
      "groups": {
        "DMD": 3,
        "Normal": 2
      },
      "statistical_unit": "reported donor/sample",
      "inference": "voom-limma pathway scores and camera competitive tests"
    },
    "PRJNA1218493": {
      "samples": 3,
      "groups": {
        "DMD": 2,
        "Normal": 1
      },
      "statistical_unit": "reported sample",
      "inference": "directional sensitivity only because one control is available"
    },
    "GSE156497": {
      "samples": 2,
      "groups": {
        "DMD_like": 1,
        "WT": 1
      },
      "species": "mouse",
      "statistical_unit": "reported sample",
      "inference": "descriptive cross-species direction only"
    }
  },
  "results": {
    "pathways_tested_prjna772047": 1526,
    "prjna772047_formal_disease_pathways": 0,
    "prjna772047_camera_supported_disease_pathways": 291,
    "disease_direction_consistent_all_3_datasets": 810,
    "strict_independent_reversal_by_background": {
      "dup2": 0,
      "dup2_9": 0,
      "dup8_9": 0
    },
    "camera_supported_independent_reversal_by_background": {
      "dup2": 48,
      "dup2_9": 4,
      "dup8_9": 0
    },
    "camera_supported_independent_reversal_2plus_backgrounds": 1,
    "camera_supported_independent_reversal_all_3_backgrounds": 0,
    "strict_independent_reversal_2plus_backgrounds": 0,
    "strict_independent_reversal_all_3_backgrounds": 0
  },
  "claim_boundary": {
    "allowed": "Sample-level external disease pathway effects and directionally opposed GSE272233 DMD-locus correction responses.",
    "blocked": "Candidate-gene perturbation response in DMD cells, therapeutic efficacy, or cell-level pseudo-replication.",
    "caveats": [
      "PRJNA772047 is small-n (3 DMD, 2 Normal).",
      "PRJNA1218493 has one control and is sensitivity-only.",
      "GSE156497 is mouse and one-versus-one; uppercase symbol matching is a heuristic orthology bridge.",
      "Pathway scores are cohort-standardized; cross-cohort effect magnitudes are not directly comparable."
    ]
  },
  "resource_release": "v1.2.0-measured-dmd-evidence",
  "interface_build": "EA-20260804-49"
}