{
  "engine_schema": "nmd-vcell-virtual-cell-engine/1.0",
  "interface_build": "EA-20260730-34",
  "resource_release": "v1.0.0-database-resource",
  "product_state": "EVIDENCE_AWARE_QUESTION_COMPOSER_NO_DMD_MODEL_INFERENCE",
  "input_contract": {
    "cell_states": [
      "proliferating_myoblast",
      "differentiating_myoblast",
      "fusing_myocyte",
      "maturing_myotube",
      "fap_stromal",
      "macrophage_immune",
      "endothelial_vascular"
    ],
    "disease_contexts": [
      "DMD",
      "healthy_or_corrected_reference",
      "DMD_vs_matched_control"
    ],
    "modalities": [
      "CRISPRi",
      "knockout",
      "CRISPRa",
      "drug",
      "pair_or_combination"
    ],
    "released_example_targets": [
      "ZNF133",
      "MON1A",
      "GFOD2"
    ],
    "time_windows": [
      "6_to_12_hours",
      "24_to_48_hours",
      "4_to_7_days"
    ],
    "endpoints": [
      "molecular_response",
      "cell_state_transition",
      "muscle_function",
      "toxicity_viability",
      "cell_cell_consequence"
    ]
  },
  "computability_layers": [
    {
      "layer": "context",
      "state": "REFERENCE_AVAILABLE",
      "substrate": "GSE52529",
      "boundary": "Unperturbed human-myogenic time course; not a DMD perturbation-conditioned trajectory."
    },
    {
      "layer": "perturbation",
      "state": "AVAILABLE_CONTEXT_BOUNDED",
      "substrate": "NMDVCELL-HEPG2-CRISPRI",
      "boundary": "Observed HepG2 CRISPRi responses for 21 candidates; no disease transport claim."
    },
    {
      "layer": "model",
      "state": "LIMITED_EXECUTED",
      "substrate": "ridge-safe-v2.3",
      "boundary": "Target-held-out recovery inside one processed assay only."
    },
    {
      "layer": "dmd_truth",
      "state": "LOCKED_NO_INDEPENDENT_OUTCOMES",
      "substrate": null,
      "boundary": "No registered independent DMD candidate perturbation outcomes exist."
    }
  ],
  "interface_behavior": {
    "executes_model_inference": false,
    "creates_server_record": false,
    "output_type": "evidence_availability_and_decision_gap",
    "permitted_interpretation": "The engine identifies which released evidence and evaluation layers can answer a composed question and which required layer is missing."
  },
  "forbidden_interpretations": [
    "calibrated DMD perturbation response",
    "therapeutic efficacy",
    "patient-specific prediction",
    "clinical recommendation",
    "cell-cell causal effect"
  ],
  "run_schema": "/resource/api/v1.1/virtual_cell_run.schema.json",
  "run_template": "/resource/downloads/virtual_cell_run_template.json"
}