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"PILOT_ARTIFACT_ONLY", "size_bytes": 1729, "sha256": "8fe018f042c9b0b6dde122a62fb300eecaef5dadb60f7cc97ed08c30c8aecc78" }, { "role": "pilot_detection_summary", "path": "GFOD2_DMD_standalone/results/server_pilot/gse288958_gfod2_detection_by_sample_celltype.tsv", "state": "PILOT_ARTIFACT_ONLY", "size_bytes": 6754, "sha256": "79e8daaeaef44473df1ae6af519e507939166e12bb2fcfd32cb530ef27ff519b" } ], "required_portable_objects": [ "authoritative source matrix or H5AD/RDS with a portable checksum", "sample metadata with donor, disease, biopsy site, age and batch", "opened cell annotation object with inherited-label provenance and an independent disagreement audit", "source software version and reference-build statement" ], "open_optional_promotion_objects": [ "unfiltered droplet matrix or author contamination estimate for ambient-RNA closure", "exact author command, sample sheet, read structure, whitelist, reference checksum and FASTQ aggregation manifest", "subject-level identity and batch metadata for future disease-effect inference", "spatial coordinates/object if any future spatial claim is retained" ], "acceptance_gates": [ "The authoritative object opens from a workspace-local path without external-share dependencies.", "Cell identifiers, sample identifiers and matrix dimensions reconcile.", "Disease, sample/biopsy unit, site and age fields are explicit; unavailable subject identity and batch remain declared.", "Independent donor-wise doublet sensitivity and marker-label disagreement audits are checksum-addressed before reuse.", "Pilot compartment selection remains descriptive and does not become a disease-effect test." ], "claim_ceiling": "Supports donor-resolved disease-state and cell-context validation only after portable import. It does not establish GFOD2 causality, therapeutic validity or DMD candidate perturbation truth.", "direct_dmd_candidate_perturbation_truth": false } ], "current_decision": "The authoritative RDS is workspace-local, checksum-verified, opened and reconciled to 59,222 cells. Independent donor-wise Scrublet and fixed-marker annotation audits are complete as bounded sensitivity/disagreement layers, so the read-only sample-aware context route is ready. Ambient RNA and author-exact reprocessing remain unclaimed." }