{ "decision_card_schema": "nmd-vcell-decision-card-set/1.0", "interface_build": "EA-20260817-57", "resource_release": "v1.2.0-measured-dmd-evidence", "ordering_policy": "Grouped by decision type and readiness; no target score, winner or numeric rank is generated.", "counts": { "decision_cards": 3, "registered_studies": 0, "registered_predictions": 0, "measured_outcomes": 0 }, "records": [ { "decision_id": "DECISION:DMD:MYOGENIC:ZNF133-MON1A:1.0-DRAFT", "title": "Resolve whether ZNF133 or MON1A has a disease-context myogenic effect", "short_title": "ZNF133 + MON1A myogenic test", "decision_type": "discriminating_experiment", "lifecycle_state": "DRAFT_NOT_REGISTERED", "state": "myoblast_to_fusing_myocyte", "targets": [ "ZNF133", "MON1A" ], "question": "Do ZNF133 or MON1A perturbations change a prespecified myogenic functional endpoint differently in DMD and matched-control cells?", "observed": [ "Observed CRISPRi response exists in HepG2 for ZNF133 (84 cells) and MON1A (94 cells).", "Both targets were assessed in the GSE293514 human-myoblast fusion screen and neither met its FDR < 0.1 hit threshold.", "Both current candidate records retain source-conflicted DMD direction and recommend a muscle-context assay." ], "decision_blocking_gap": "Neither target has an independent perturbation repeated in a DMD muscle model.", "next_action": "Compare both targets in the same matched DMD/control human-myogenic assay, using qualified independent reagents and one frozen primary functional endpoint.", "result_changes": [ "A reproducible DMD-specific effect would justify disease-context follow-up.", "A similar effect in DMD and control would support a broader myogenic—not DMD-specific—interpretation.", "A near-zero result inside a frozen equivalence margin would downgrade the hypothesis.", "Viability loss, differentiation blockade or reagent discordance would classify the result as toxic or inconclusive." ], "evidence_links": [ { "label": "Compare ZNF133 and MON1A", "href": "/resource/compare?genes=ZNF133,MON1A" }, { "label": "Open ZNF133 evidence", "href": "/resource/gene/ZNF133" }, { "label": "Design the experiment", "href": "/resource/planner?gene=ZNF133" } ], "claim_boundary": "This card recommends a discriminating experiment. It does not predict that either target will improve DMD." }, { "decision_id": "DECISION:EVIDENCE-AUDIT:GFOD2:1.0-DRAFT", "title": "Determine whether pending external evidence should change the GFOD2 record", "short_title": "GFOD2 evidence audit", "decision_type": "evidence_import_and_audit", "lifecycle_state": "PROVISIONAL_HOLD_IMPORT_PENDING", "state": "evidence_provenance", "targets": [ "GFOD2" ], "question": "Does the author-reported external assessment provide traceable quantitative evidence that survives the released import and audit rules?", "observed": [ "GFOD2 has an observed HepG2 CRISPRi response with 127 cells in the frozen aggregate.", "GFOD2 was not included in the GSE293514 human-myoblast fusion library.", "Its historical rank is preserved only for reproducibility; there is no current numeric rank." ], "decision_blocking_gap": "The reported external quantitative evidence, source provenance and analysis code have not been imported and audited.", "next_action": "Import the external result with source identifiers, quantitative values and analysis provenance, then run the released validation contract before changing status.", "result_changes": [ "A complete, reproducible import can move the record from provisional hold to computational triage.", "An incomplete or incompatible import leaves the record on hold.", "Either outcome remains contextual evidence—not DMD perturbation efficacy." ], "evidence_links": [ { "label": "Open GFOD2 evidence", "href": "/resource/gene/GFOD2" }, { "label": "Read the evidence transition", "href": "/resource/evidence_transition" }, { "label": "Download the import template", "href": "/resource/downloads/external_candidate_evidence_import_template.tsv" } ], "claim_boundary": "Audit resolution can change evidence status but cannot create a muscle, DMD or therapeutic validation claim." }, { "decision_id": "DECISION:DMD:MINIMUM-PERTURBOME:24-SLOT:1.0-DRAFT", "title": "Freeze a balanced 24-slot DMD pilot before any outcome is visible", "short_title": "24-slot pilot freeze", "decision_type": "prospective_program_freeze", "lifecycle_state": "DRAFT_PANEL_ASSIGNED_SIGNOFF_PENDING", "state": "program_design", "targets": [], "question": "Which candidate, control and calibration conditions should occupy the 24 pilot slots under rules frozen before outcome access?", "observed": [ "All 24 Stage A identities are provisionally assigned: 14 candidate genes and 10 calibration controls.", "The assignments remain editable; panel signoff and a frozen checksum are still pending.", "No study or prediction has been registered, no experiment has started and no outcome has been measured." ], "decision_blocking_gap": "Panel signoff, one primary endpoint, a minimally important effect and a negligible-effect margin remain unfrozen.", "next_action": "Sign off and checksum-freeze the assigned panel, endpoint and decision thresholds, then register an immutable truth-generation Study. Add a Prediction only when a condition is explicitly assigned to prediction validation.", "result_changes": [ "A frozen panel makes future hit-rate and enrichment claims auditable.", "Controls allow calibration of false positives, nulls and toxicity.", "Pre-outcome registration prevents retrospective relabelling of successes and failures." ], "evidence_links": [ { "label": "Open the 24-slot protocol", "href": "/resource/minimum-perturbome/" }, { "label": "Review the no-wet-lab pack", "href": "/resource/no-wet-lab-evaluation/" }, { "label": "Open the empty registry", "href": "/resource/registry" } ], "claim_boundary": "This is an assigned but unfrozen prospective design. It contains no registered experiment, measured outcome or efficacy result." } ], "claim_boundary": "Decision Cards identify evidence-resolving actions. They are not treatment recommendations or calibrated DMD perturbation predictions." }